Continuous Flow chips must be calibrated whenever you want to use them with reagents that have not already been calibrated for continuous flow use. Continuous Flow chip calibration determines the amount of fluid dispensed per second.
Go to the Tools menu and select Continuous Flow Calibration.
Next to Reagent, select the reagent you want to use with the Continuous Flow chip.
Next to Chip, select HV Continuous Flow.
Next to Dispense Time, type the number of seconds you want the MANTIS to dispense the reagent for. If you plan to dispense 200 µL, 1 second is enough. For a 650 µL dispense, you should run a 3 second test. You can type in fractions of a second if you would like to calibrate for smaller volumes.
Follow the on-screen instructions.
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